Multiplex TEMPase Master Mix is developed for the simultaneous amplification of two or more amplicons in a single tube. Multiplex TEMPase Master Mix minimises the need for optimisation and makes the development of multiplex PCR fast and easy.
FEATURES
Amplification of multiple PCR products in one tube
Diminished formation of non-specific products
Detection of low abundance targets
Reaction set-up at room temperature
High sensitivity, sensitivity and product yield
DESCRIPTION
Multiplex 2x Master Mix is composed of TEMPase Hot Start DNA Polymerase and a specialised buffer system designed for multiplex PCR. TEMPase Hot Start DNA Polymerase is well suited for multiplex PCR due to its high specificity. MgCl2 is enclosed in the multiplex kit to enable optimisation.
AMPLIFICATION OF A FIVE-PLEX AND A TEN-PLEX REACTION
Five different templates of the CFTR gene (5-plex) and ten different templates of the DMD gene (10-plex) were amplified simultaneously in one tube, respectively. M: Marker.
