When do you use TEMPase Hot Start DNA Polymerase?
TEMPase Hot Start has been designed to avoid spurious
amplification and to diminish the formation of non specific priming
events during your set up. TEMPase Hot Start allows for detection
of low target copy numbers and for multiplex PCR in a single
reaction tube.
Features
- Reaction set up at room temperature
Increased
- Sensitivity
- Specificity
- Product yield
Description
TEMPase Hot Start DNA Polymerase is a modified form of Ampliqon
Taq DNA Polymerase, which is activated by heat treatment.
A chemical moiety is attached to the enzyme at the active site,
which renders the enzyme inactive at room temperature. Thus, during
set up and the first ramp of thermal cycling, the enzyme is not
active and misprimed primers are not extended.
Once the reaction reaches optimal activating temperature, the
chemical moiety is cleaved during a heat activation step, releasing
the active TEMPase Hot Start DNA Polymerase into the reaction.
