When do you use TEMPase Hot Start Master Mix Blue?

The blue loading dye facilitates direct gel loading and eliminates the need for separate loading dye for electrophoresis and visualization after PCR. TEMPase Hot Start 2 x Master Mix Blue is an alternative to TEMPase Hot Start 2 x Master Mix with extra time saving advantages.

Features

  • Inactive at room temperature
  • Premixed 2 x solution

Description

Ampliqon TEMPase Hot Start DNA Polymerase, dNTPs, a blue loading dye and magnesium chloride are present in TEMPase Hot Start 2 x Master Mix with TEMPase Buffer I (balanced KCl / (NH4)2 SO4 buffer system)  or TEMPase Buffer II ((NH4)2 SO4 buffer system).

The two TEMPase buffers cover all PCR applications and allow your choice of buffer to be determined by your amplification needs.

TEMPase Hot Start 2 x Master Mix Blue_fig1.jpg