TEMPase Hot Start DNA Polymerase 2x Master Mix BLUE is a time saving alternative to TEMPase Hot Start DNA Polymerase 2x Master Mix. It offers the same excellent performance. In addition, the blue loading dye facilitates direct gel loading. You do not need a separate loading buffer and time-consuming sample preparation before electrophoresis. This makes TEMPase Hot Start DNA Polymerase 2x Master Mix BLUE especially suitable for high throughput testing.
FEATURES
Minimal optimisation
Time-saving premixed solution
Direct loading onto agarose and SDS DNA gels
Easy visualisation of pipetting
Dye front running at 100-500 bp (0.5 -1.5 % agarose gel)
Convenient reaction set-up at room temperature
Increased sensitivity, specificity and product yield
Consistent test results
Lower risk of contamination
DESCRIPTION
TEMPase Hot Start DNA Polymerase 2x Master Mix BLUE is a ready-to-use 2x master mix composed of dNTPs, a blue loading dye, a stabiliser, MgCl2 and either TEMPase Buffer C (a balanced KCl / (NH4)2 SO4 buffer system) or TEMPase Buffer A (a (NH4)2 SO4 buffer system).
The blue dye and stabiliser do not interfere with the PCR. If necessary, the blue dye can be removed by spin column purification or other methods. Furthermore, PCR products generated using TEMPase Hot Start DNA Polymerase 2x Master Mix BLUE, can directly be used for sequencing after treatment with either spin columns or PureIT ExoZAP PCR CleanUp.
TIP - CHOOSE THE RIGHT TEMPASE HOT START MASTER MIX
For most standard applications 2x Master Mix BLUE A based on Ammonium Buffer is the best option. It promotes robust amplification, high yield and high specificity. In certain cases Master Mix BLUE C is the best option.
DIRECT GEL LOADING
After end of amplification, using TEMPase DNA Polymerase Hot Start 2x Master Mix BLUE PCR products can be loaded directly onto agarose and SDS DNA gels. The blue dye provides easy visualisation of both pipetting and gel loading. The blue dye front runs at 100 - 500 bp on 0.5 - 1.5 % agarose gels.
